Assays
Pre-validated, automated workflows for reproducible quantification across every assay your lab runs. No coding, no GPU, no software to install.
Immune & Uptake Assays
Antibody Binding Efficiency
Quantifies surface-bound antibody fluorescence per cell across concentrations to compare antibody clones, isotypes, or conjugation strategies.
Bacteria Internalization
Quantifies per-cell bacterial infection load by integrating the bacterial channel intensity inside each segmented host cell.
Endocytosis & Peptide Uptake
Quantifies cellular uptake of fluorescently labeled compounds across concentrations.
Immune-Mediated Target-Cell Killing
Quantifies endpoint killing of a labeled target-cell population by immune effector cells in co-culture.
Macrophage Polarization Phenotyping
Quantifies marker-defined macrophage activation states using multiplex fluorescence and morphology.
NET Analysis
Detects and quantifies neutrophil extracellular traps (NETs) by measuring the expanded DNA/protein meshwork released from activated neutrophils.
Neutralisation Assay
Quantifies the ability of antibodies to block pathogen infection by scoring infected vs uninfected cells across antibody concentrations.
Parasite Infection
Quantifies parasite infection of host cells by measuring the fraction of infected cells and the parasite burden per cell.
Phagocytosis
Quantifies the engulfment of prey particles by phagocytic cells.
T-Cell Activation & Exhaustion Markers
Measures the fraction of T cells expressing activation, proliferation, or exhaustion-associated markers in endpoint images.
Viral Internalization
Quantifies viral particle uptake by host cells, analogous to phagocytosis.
Cell Growth, Survival & Fitness
Apoptosis (Annexin V / PI)
Classifies cells into viable, early apoptotic, late apoptotic, and necrotic populations using dual-marker staining and quadrant gating. Also supports endpoint apoptosis markers such as TUNEL (DNA-fragmentation) and cleaved/active caspase-3.
BrdU/EdU Proliferation
Measures the fraction of cells actively synthesizing DNA using nucleotide analog incorporation.
Cell Counting & Viability
Counts total cells and classifies them as live or dead based on marker intensity, providing the most fundamental readout for toxicity and dose-response experiments.
Cell Cycle Analysis
Categorizes cells into G0/G1, S/G2, and M phases using cell morphology and DNA stain intensity, preserving spatial context unlike flow cytometry.
Clonogenic Survival & Colony Formation
Counts discrete colonies and measures colony size and morphology to quantify reproductive survival after treatment.
Autophagy & Vesicle Trafficking
Autophagy & Mitophagy Flux
Detects and quantifies autophagic puncta per cell, with optional ratiometric classification of puncta maturation state.
Biomolecular Condensates & Phase Separation
Quantifies the formation, size, and intensity of liquid-liquid phase-separated condensates in cells.
Lysosomal pH Activity
Quantifies lysosomal activity by combining a lysosomal marker with a pH-sensitive dye to score the fraction of acidic lysosomes per cell.
Lysosome & Endosome Abundance
Counts and measures lysosomal or endosomal compartments per cell as an endpoint abundance and morphology assay.
Cell Identity, Expression & Population Analysis
Blood Cell Classification
Classifies blood cell types using nuclear and cytoplasmic markers with interactive scatter plot gating, analogous to flow cytometry but with spatial context preserved.
Gene Knockdown & Overexpression Validation
Validates loss or gain of target-protein expression by measuring per-cell fluorescence and the fraction of responding cells.
Mixed-Culture Composition
Quantifies the proportions and phenotypes of two or more marker-defined cell populations in co-culture.
Multiplex Imaging Analysis
Simultaneously quantifies multiple markers across four or more channels on a per-cell basis for multi-parametric phenotyping.
Rare Phenotype Detection by Gating
Finds uncommon cells defined by marker intensity and morphology combinations and links them back to their images.
Sample Purity & Quality Control
Identification and quantification of contaminants in cell preparations
Stem Cell Lineage & Differentiation QC
Quantifies differentiation efficiency by measuring the fraction of cells expressing lineage-specific markers.
Transfection Efficiency
Quantifies the fraction of cells expressing one or more transfected constructs.
Cell Behaviour, Adhesion & Migration
Cell Adhesion & Retention
Measures the number and morphology of cells retained after attachment, washing, or mechanical challenge at a fixed endpoint.
Transwell / Boyden Migration & Invasion
Counts and measures cells that have migrated through a porous membrane, with or without a matrix barrier.
Wound Healing & Edge Polarisation — Endpoint
Quantifies the remaining wound area and the orientation or polarisation of cells at a wound edge from endpoint images.
Cell Morphology & Phenotypic Profiling
Cell Hypertrophy & Size Response
Quantifies treatment- or differentiation-associated changes in cell and nuclear size at single-cell resolution.
Cell Morphology & Phenotypic Profiling
Measures the size, shape, and fluorescence intensity of individual cells across multiple markers.
Cell Painting–Style Morphological Profiling
Extracts multiparametric endpoint morphology, intensity, and sub-object measurements from Cell Painting–style images.
Epithelial–Mesenchymal Transition Phenotyping
Classifies epithelial, mesenchymal, and intermediate cell states using marker expression and simple cell morphology.
Multi-Nuclei Detection
Identifies multinucleated cells within a population for downstream analysis or targeted re-acquisition at higher magnification.
Tissue & Structural Analysis
Cell Proximity & Neighbourhood Analysis
Uses cell coordinates and marker-defined identities to quantify proximity, neighbourhood composition, clustering, and infiltration in images.
Chromogenic IHC Positivity & H-Score
Quantifies chromogenic IHC staining by classifying cells into negative, weak, moderate, and strong intensity groups.
Filament Alignment
Quantifies the orientation and alignment coherency of fibrous structures within cells.
Muscle Fiber Nuclear Localization
Detects and classifies nuclear positioning within muscle fibers to assess muscle damage and regeneration.
Positive-Cell Density in Tissue
Counts marker-positive cells in a 2D tissue section and reports positive-cell fraction and density per area.
Tissue Cellularity Quantification
Counts nuclei and cells across tissue sections from a nuclear counterstain, reporting cellularity per region with full spatial context.
DNA Damage & Senescence
Cellular Senescence (SA-β-gal) & Morphometry
Identifies senescent cells and characterizes their phenotypic features including enlarged morphology, SA-β-gal positivity, and DNA damage markers.
Cytosolic Nucleic Acid Sensing (Innate Immunity)
Detects cytoplasmic DNA or cGAS/STING puncta as markers of innate immune activation.
DNA Damage & Genotoxicity (Foci)
Quantifies DNA double-strand break burden by counting damage-response foci per nucleus.
Micronucleus & Cytokinesis-Block Genotoxicity
Detects micronuclei and related nuclear abnormalities in mononucleated or cytokinesis-blocked binucleated cells.
Protein Localization & Colocalization
Endpoint Receptor Internalization
Compares membrane-associated and intracellular receptor signal at a fixed endpoint to quantify internalization.
Membrane Receptor Binding
Quantifies agonist or antibody binding to membrane-localized receptors by isolating membrane signal from cytoplasmic background.
Nuclear Translocation
Measures the redistribution of a protein between nucleus and cytoplasm in response to a stimulus (e.g., YAP, NF-κB, FOXO, p65).
Protein Co-localization & Co-expression
Determines whether two proteins or markers occupy the same cellular regions, using pixel-level correlation within each cell. Used to assess receptor-ligand interactions, organelle contact sites, pathway co-activation, and drug target engagement.
Metabolic & Oxidative Stress
Lipid Droplet Quantification
Detects and measures lipid droplets within cells to quantify lipid accumulation.
Lipid Peroxidation & Oxidative Stress (ROS)
Measures cellular oxidative burden via fluorescent ROS indicators.
Mitochondrial Fragmentation & Mass
Measures mitochondrial object count, size distribution, total area, and fluorescence per cell without reconstructing the full network.
Mitochondrial Membrane Potential — Endpoint
Measures mitochondrial polarization or depolarization from endpoint TMRM, TMRE, or JC-1 fluorescence images.
Puncta, Foci & Sub-Object Analysis
Multi-Marker Foci Association
Detects puncta in two channels independently and scores their association per cell via count-based colocalization. Used to determine whether foci of one type co-occur with foci of another type within the same cells.
Spot & Foci Detection
Detects, counts, and measures small bright puncta within cells.
Sub-Object Quantification
Counts and measures smaller objects contained within larger parent objects, then aggregates statistics per parent. This enables quantification of organelles per cell, inclusions per nucleus, or vesicles per compartment.
Need a custom assay workflow?
Every lab is different. Our image analysis team will build a tailored workflow for your specific assay at no extra cost.