Chromogenic IHC Positivity & H-Score

Quantifies chromogenic IHC staining by classifying cells into negative, weak, moderate, and strong intensity groups.

Chromogenic IHC Positivity & H-Score illustration
Input channels
  • Brightfield image with hematoxylin counterstain
  • Chromogenic target stain such as DAB
Output metrics
  • % positive cells
  • % negative, weak, moderate, and strong cells
  • Intensity per cell
  • H-score or intensity-weighted positivity

Everything you need for chromogenic ihc positivity & h-score

Automated, reproducible, and code-free analysis in the cloud

Cloud-based

No software to install, no GPU required. Run analysis from any browser on any device.

Fully automated

Upload your images and get publication-ready results. No coding or scripting needed.

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Every analysis is logged and reproducible. Share protocols with collaborators instantly.

How it runs

Cytely automates the image analysis so you can focus on the science.

01

Separate nuclear counterstain and chromogen

02

Segment nuclei and optional cell compartments

03

Measure chromogen intensity per cell

04

Classify intensity bins

05

Compute positivity and H-score.

Compatibility

Ready to run

DAB/hematoxylin IHC, nuclear-marker scoring, membrane or cytoplasmic positivity where segmentation is reliable, tissue microarray cores analysed individually.

Inquire for support

Pathologist-grade tumour-region annotation, diagnostic grading, complex histological pattern recognition without dedicated validation.

1 Dependent on sample quality

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