Cell Proximity & Neighbourhood Analysis
Uses cell coordinates and marker-defined identities to quantify proximity, neighbourhood composition, clustering, and infiltration in images.
- Ch1: DAPI, Hoechst, or hematoxylin
- Ch2-4: markers used to classify cell populations
- Optional annotated tissue boundary or region mask
- Nearest-neighbour distance by cell-type pair
- Number and composition of cells within a defined radius
- Homotypic and heterotypic clustering metrics
- Distance to tissue, tumour, or compartment boundary
- 2D infiltration depth and density by region
Everything you need for cell proximity & neighbourhood analysis
Automated, reproducible, and code-free analysis in the cloud
Cloud-based
No software to install, no GPU required. Run analysis from any browser on any device.
Fully automated
Upload your images and get publication-ready results. No coding or scripting needed.
Reproducible
Every analysis is logged and reproducible. Share protocols with collaborators instantly.
How it runs
Cytely automates the image analysis so you can focus on the science.
Segment and classify cells
Retain centroid coordinates
Optionally define tissue regions or boundaries
Calculate pairwise distances and radius-based neighbourhoods
Summarize by population and condition.
Compatibility
Ready to run
Tumour–immune proximity, immune-cell clustering, mixed-culture neighbourhoods, core-versus-margin analysis, basic niche composition, marker-positive cell proximity in conventional multiplex images.
Inquire for support
Full CODEX/PhenoCycler/IMC high-plex workflows, cyclic image registration, advanced graph-community inference, or 3D neighbourhood analysis. This proposal assumes coordinate-derived measurements can be exposed reliably in the product, volumetric 3D neighbourhood analysis.
1 Dependent on sample quality
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The data-driven approach that Cytely offers is a dream to an experienced microscopist like me.
This completely removes bias. The price is negligible when you consider the time spent on image analysis.
It's just amazing what can be done today! Cytely does all the work for me!
Cytely makes me 10 times faster. It saves hours on every experiment.
The insight we gained from Cytely was a completely transformative experience. It allowed us to redesign our experiment and finally move the project forward.
Cytely dramatically improved our timelines while enhancing data reliability through automated, unbiased analysis and significantly larger sample sizes.
With Cytely, we instantly analyzed thousands of cells to precisely identify which were transfected, infected, or both. What had been a three-year roadblock was resolved in our first session.
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