Positive-Cell Density in Tissue

Counts marker-positive cells in a 2D tissue section and reports positive-cell fraction and density per area.

Positive-Cell Density in Tissue illustration
Input channels
  • Ch1: DAPI, Hoechst, or hematoxylin
  • Ch2: fluorescent or chromogenic marker
  • Optional Ch3-4: cell-type or compartment markers
Output metrics
  • Positive-cell count
  • % marker-positive cells
  • Positive-cell density per unit area
  • Marker intensity per cell

Everything you need for positive-cell density in tissue

Automated, reproducible, and code-free analysis in the cloud

Cloud-based

No software to install, no GPU required. Run analysis from any browser on any device.

Fully automated

Upload your images and get publication-ready results. No coding or scripting needed.

Reproducible

Every analysis is logged and reproducible. Share protocols with collaborators instantly.

How it runs

Cytely automates the image analysis so you can focus on the science.

01

Segment nuclei or whole cells

02

Measure marker intensity per object

03

Classify positive and negative cells by threshold or gating

04

Report counts, fractions, and density per area.

Compatibility

Ready to run

Ki-67 index, cleaved-caspase-3 density, TUNEL-positive density, immune-marker-positive density, lineage-marker positivity in FFPE or frozen sections.Projection-assisted counting may be used for shallow, sparsely labelled stacks where overlap across depth is limited.

Inquire for support

Automated anatomical region segmentation, complex tumour–stroma neighbourhood analysis, 3D tissue density.Projected density remains area-based rather than volumetric and can undercount overlapping cells.

1 Dependent on sample quality

Ready to automate your positive-cell density in tissue analysis?

Get started for free or book a demo to see how Cytely works with your data.

Trusted by leading research institutions

Hundreds of researchers use Cytely to accelerate discovery

Simple, transparent pricing

Start free. Upgrade when you need more.

Free

For exploration and learning

$0

Free forever

  • 1 monthly analysis credit
  • Unlimited public datasets
  • Community support
Get started

Individual

For active researchers

From $299

per month

  • 10+ monthly analysis credits
  • Image analysis support
  • Priority email support
See pricing

Lab / Team

For collaborating teams

Custom

Contact us

  • 5+ seats included
  • Shared folders and templates
  • Dedicated support
Book demo

Loved by researchers

Hear from researchers who use Cytely every day.

Chief Scientific OfficerEuropean Contract Research Organization

The data-driven approach that Cytely offers is a dream to an experienced microscopist like me.

Professor in Muscle BiologyLeading European Research University

This completely removes bias. The price is negligible when you consider the time spent on image analysis.

Hematology ResearcherHematology Researcher

It's just amazing what can be done today! Cytely does all the work for me!

Infection Medicine ResearcherDoctoral Student, Lund University

Cytely makes me 10 times faster. It saves hours on every experiment.

Alex Evilevitch
Alex EvilevitchProfessor, Lund University

The insight we gained from Cytely was a completely transformative experience. It allowed us to redesign our experiment and finally move the project forward.

SwathiCancer Mechanobiology Researcher

Cytely dramatically improved our timelines while enhancing data reliability through automated, unbiased analysis and significantly larger sample sizes.

Blazej Cegielski
Blazej CegielskiVirus Biophysics Lab

With Cytely, we instantly analyzed thousands of cells to precisely identify which were transfected, infected, or both. What had been a three-year roadblock was resolved in our first session.

Curious about this assay?

Book a quick call and our team will help you set up your analysis.

Explore more assays

Other assay needs?

Talk to our image analysis specialists. We're adding new assays regularly.