Biomolecular Condensates & Phase Separation
Quantifies the formation, size, and intensity of liquid-liquid phase-separated condensates in cells.
- Ch1: DAPI
- Ch2: condensate marker (fluorescent tag or IF for scaffold protein, e.g., G3BP1 for stress granules, FUS, TDP-43)
- Condensate count per cell
- Mean condensate area
- Total condensate area fraction
- Condensate intensity
Everything you need for biomolecular condensates & phase separation
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Segment cells
Detect condensates as puncta in marker channel
Measure count, area, and intensity per cell.
Compatibility
Supported
Stress granules (G3BP1), P-bodies (DCP1A), PML bodies, nucleolar markers, any punctate phase-separated structure.
Not yet supported
Condensate dynamics (FRAP recovery), 3D condensate volume.
1 Dependent on sample quality
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