Autophagy & Mitophagy Flux
Detects and quantifies autophagic puncta per cell, with optional ratiometric classification of puncta maturation state.
- Ch1: DAPI
- Ch2: autophagy reporter (LC3, p62, LAMP1 IF or GFP-LC3 tag). For flux: Ch2+Ch3 from a dual-reporter (mCherry-GFP-LC3 or mt-Keima at two excitation wavelengths)
- Puncta count per cell
- Puncta area
- Puncta intensity. For flux: ratio of Ch2/Ch3 intensity per punctum to classify acidic vs neutral
Everything you need for autophagy & mitophagy flux
Automated, reproducible, and code-free analysis in the cloud
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How it runs
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Segment nuclei/cells
Detect puncta in reporter channel
Count and measure per cell.
Compatibility
Supported
Single-channel puncta counting (LC3, p62, LAMP1), bafilomycin flux (puncta count difference ± treatment).
Not yet supported
Ratiometric puncta classification (mt-Keima 458/561nm, mCherry-GFP-LC3 red-only vs yellow) — minor platform addition needed.
1 Dependent on sample quality
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