Membrane Receptor Binding

Quantifies agonist or antibody binding to membrane-localized receptors by isolating membrane signal from cytoplasmic background.

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Input channels

Ch1: cell body / nuclear stain
Ch2: membrane marker or labeled ligand
Optional Ch3: additional marker

Output metrics

Membrane-associated intensity per cell
Membrane-to-cytoplasm intensity ratio

Everything you need for membrane receptor binding

Automated, reproducible, and code-free analysis in the cloud

Cloud-based

No software to install, no GPU required. Run analysis from any browser on any device.

Fully automated

Upload your images and get publication-ready results. No coding or scripting needed.

Reproducible

Every analysis is logged and reproducible. Share protocols with collaborators instantly.

How it runs

Cytely automates the image analysis so you can focus on the science.

Segment cells

Create membrane compartment mask by isolating the cell periphery

Measure intensity in membrane compartment separately from cytoplasm

Compute compartment ratio.

Compatibility

Ready to run¹

Labeled agonist binding, fluorescent antibody surface staining, any membrane-localized signal.

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Binding curves across concentrations, receptor internalization kinetics, single-molecule receptor tracking.

¹ Dependent on sample quality

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Publication

2026

Characterization of white adipocyte heterogeneity using data-driven microscopy

Zhang J, Simonsson C, Fawad A, Alsalim W, Vaag A, Neuhaus M, Stenkula KG. International Journal of Obesity

Using Cytely's data-driven microscopy platform, researchers profiled hundreds of individual human fat cells and found that many co-express high levels of the subtype markers DDR2 and PLIN1 alongside consistent GLUT4 levels, evidence for distinct mature adipocyte subtypes and a scalable way to validate single-cell heterogeneity first identified by transcriptomics.

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