Endpoint Receptor Internalization
Compares membrane-associated and intracellular receptor signal at a fixed endpoint to quantify internalization.
- Ch1: DAPI or cell mask
- Ch2: labeled receptor, ligand, or antibody
- Optional Ch3: surface-only counterstain or endosomal marker
- Membrane-associated intensity
- Intracellular receptor intensity
- Membrane-to-interior ratio
- Internal receptor puncta count per cell
Everything you need for endpoint receptor internalization
Automated, reproducible, and code-free analysis in the cloud
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Upload your images and get publication-ready results. No coding or scripting needed.
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How it runs
Cytely automates the image analysis so you can focus on the science.
Segment cells
Define membrane and inner-cell compartments
Measure receptor signal in each compartment
Optionally detect intracellular receptor puncta
Classify internalized versus surface-dominant cells.
Compatibility
Ready to run
Antibody uptake, ligand-induced receptor internalization, transferrin-receptor endpoint assays, surface-versus-internal differential staining.
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Internalization rate constants, recycling kinetics, single-vesicle tracking.
1 Dependent on sample quality
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