Protein Co-localization & Co-expression
Determines whether two proteins or markers occupy the same cellular regions, using pixel-level correlation within each cell. Used to assess receptor-ligand interactions, organelle contact sites, pathway co-activation, and drug target engagement.
- Ch1: DAPI
- Ch2: marker A (IF or fluorescent tag)
- Ch3: marker B (IF or fluorescent tag)
- Pearson correlation coefficient per cell
- Manders M1/M2 overlap coefficients per cell
- % cells with positive colocalization
- Channel intensity ratios per cell
Everything you need for protein co-localization & co-expression
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Segment cells
Compute Pearson correlation and Manders coefficients between Ch2 and Ch3 within each cell. Optionally restrict analysis to a specific compartment (nucleus-only or cytoplasm-only).
Compatibility
Supported
Receptor-ligand co-localization (e.g., EGFR + EGF), organelle contact sites (e.g., mitochondria-ER via MitoTracker + ER-Tracker), pathway co-activation (e.g., phospho-protein + total protein), drug accumulation localization (e.g., compound + lysosomal marker), compartment-specific colocalization (nuclear-only, cytoplasmic-only).
Not yet supported
Object-based colocalization (spot-to-spot spatial overlap scoring), cross-correlation function analysis, 3D colocalization.
1 Dependent on sample quality
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