Antibody Binding Efficiency
Quantifies surface-bound antibody fluorescence per cell across concentrations to compare antibody clones, isotypes, or conjugation strategies.
- Ch1: DAPI
- Ch2: fluorescently labeled antibody signal
- Mean surface fluorescence per cell
- Binding intensity distribution
- Binding curves across concentrations
Everything you need for antibody binding efficiency
Automated, reproducible, and code-free analysis in the cloud
Cloud-based
No software to install, no GPU required. Run analysis from any browser on any device.
Fully automated
Upload your images and get publication-ready results. No coding or scripting needed.
Reproducible
Every analysis is logged and reproducible. Share protocols with collaborators instantly.
How it runs
Cytely automates the image analysis so you can focus on the science.
Segment cells
Measure antibody channel intensity per cell
Compare intensity distributions across concentrations or antibody variants.
Compatibility
Supported
Antibody clone comparison, isotype controls, conjugation strategy evaluation, any labeled antibody binding assay.
Not yet supported
Binding affinity (Kd) calculation, kinetic on/off rate measurement.
1 Dependent on sample quality
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