Endocytosis & Peptide Uptake

Quantifies cellular uptake of fluorescently labeled compounds across concentrations.

Endocytosis & Peptide Uptake illustration
Input channels
  • Ch1: DAPI
  • Ch2: fluorescently labeled peptide, toxin, or compound (e.g., pHrodo, Alexa-labeled)
Output metrics
  • Mean intracellular fluorescence per cell
  • % cells with uptake
  • Concentration-dependent uptake profiles

Everything you need for endocytosis & peptide uptake

Automated, reproducible, and code-free analysis in the cloud

Cloud-based

No software to install, no GPU required. Run analysis from any browser on any device.

Fully automated

Upload your images and get publication-ready results. No coding or scripting needed.

Reproducible

Every analysis is logged and reproducible. Share protocols with collaborators instantly.

How it runs

Cytely automates the image analysis so you can focus on the science.

01

Segment cells

02

Measure intracellular fluorescence intensity per cell

03

Generate uptake profiles across concentrations.

Compatibility

Supported

Cell-penetrating peptide uptake, cholera toxin binding and internalization, transferrin uptake, receptor-mediated endocytosis, any fluorescently labeled compound uptake assay.

Not yet supported

Endosomal escape quantification, uptake kinetics (timelapse).

1 Dependent on sample quality

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Alex EvilevitchProfessor, Lund University

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