Immune-Mediated Target-Cell Killing
Quantifies endpoint killing of a labeled target-cell population by immune effector cells in co-culture.
- Ch1: DAPI or Hoechst
- Ch2: target-cell identity marker or stable fluorescent label
- Ch3: effector-cell identity marker
- Optional Ch4: viability or death marker such as PI, 7-AAD, or activated caspase reporter
- % dead target cells
- Viable target-cell count
- Target-cell survival relative to target-only control
- Specific killing or specific lysis
- Effector-to-target ratio measured in the image
- Effector-cell viability
Everything you need for immune-mediated target-cell killing
Automated, reproducible, and code-free analysis in the cloud
Cloud-based
No software to install, no GPU required. Run analysis from any browser on any device.
Fully automated
Upload your images and get publication-ready results. No coding or scripting needed.
Reproducible
Every analysis is logged and reproducible. Share protocols with collaborators instantly.
How it runs
Cytely automates the image analysis so you can focus on the science.
Segment all cells
Classify target and effector populations from identity markers
Measure viability or apoptosis signal within target cells
Calculate target-specific killing and normalize to controls.
Compatibility
Ready to run
T-cell-mediated killing, NK-cell cytotoxicity, macrophage-mediated killing, antibody-dependent cellular cytotoxicity (ADCC), redirected killing assays, mixed-culture target survival.
Inquire for support
Serial killing, immune-synapse dynamics, contact duration, time-to-kill, or lineage-resolved killing; these require time-series tracking. Image analysis alone does not establish antibody dependence without suitable controls.
1 Dependent on sample quality
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