Phagocytosis
Quantifies the engulfment of prey particles by phagocytic cells.
- Ch1: DAPI
- Ch2: phagocyte marker or cell mask
- Ch3: labeled prey (e.g., pHrodo-labeled bacteria, fluorescent beads, synaptosomes)
- Prey count per phagocyte
- Total prey area per cell
- % phagocytosis-positive cells
- Prey intensity per cell
- Internalization scoring (surface-bound vs internalized prey via marker or 3D estimate)
- Image multiplicity of prey (iMOP — estimated prey-to-cell ratio in the sample)
Everything you need for phagocytosis
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Segment phagocytes
Detect prey particles
Measure prey count, area, and intensity per phagocyte. Internalization can be scored using a pH-sensitive dye (e.g., pHrodo, which fluoresces only when internalized) or by estimating 3D overlap. iMOP provides a control metric for normalizing phagocytic activity against prey availability.
Compatibility
Supported
Macrophage, microglial, neutrophil, or monocyte/THP-1 phagocytosis. Bead-based, bacterial, synaptosome engulfment, antibody-dependent cellular phagocytosis (ADCP), any labeled-prey assay. Internalization scoring via pH-sensitive dyes or 3D estimation.
Not yet supported
Live-cell phagocytosis kinetics, phagosome maturation tracking.
1 Dependent on sample quality
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