Bacteria Internalization
Quantifies per-cell bacterial infection load by integrating the bacterial channel intensity inside each segmented host cell.
- Ch1: DAPI (host nuclei)
- Ch2: host cell marker
- Ch3: bacterial label (GFP / mCherry / IF / pHrodo)
- Optional Ch4: extracellular-only counterstain for inside/outside discrimination
- Bacteria per host cell
- Total bacterial signal per cell
- % infected cells
- % internalized vs. adhered bacteria
Everything you need for bacteria internalization
Automated, reproducible, and code-free analysis in the cloud
Cloud-based
No software to install, no GPU required. Run analysis from any browser on any device.
Fully automated
Upload your images and get publication-ready results. No coding or scripting needed.
Reproducible
Every analysis is logged and reproducible. Share protocols with collaborators instantly.
How it runs
Cytely automates the image analysis so you can focus on the science.
Segment host cells
Detect bacteria as sub-objects
Classify inside vs. outside via differential stain
Measure count, area and intensity per cell.
Compatibility
Ready to run
GFP/mCherry-expressing strains, IF-stained fixed bacteria, pHrodo-labeled live uptake, inside/outside differential immunostaining, gentamicin-protection endpoints.
Inquire for support
Live-cell entry kinetics at sub-second resolution, intracellular bacterial replication time-courses, secreted-effector translocation assays.
1 Dependent on sample quality
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With Cytely, we instantly analyzed thousands of cells to precisely identify which were transfected, infected, or both. What had been a three-year roadblock was resolved in our first session.
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