Cellular Senescence (SA-β-gal) & Morphometry
Identifies senescent cells and characterizes their phenotypic features including enlarged morphology, SA-β-gal positivity, and DNA damage markers.
- Ch1: DAPI
- Ch2: brightfield (SA-β-gal chromogenic stain)
- Optional Ch3-4: p21, γH2AX, or other senescence markers (IF)
- % SA-β-gal positive cells
- Nuclear area
- Cell area
- Foci counts (γH2AX)
- CCF count
- Intensity per marker
Everything you need for cellular senescence (sa-β-gal) & morphometry
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Segment nuclei from DAPI
Measure SA-β-gal intensity from brightfield
Classify positive/negative by threshold. For DNA damage: detect foci in marker channel. For CCF: create cytoplasmic ring (dilate nuclei
Subtract original)
Detect DAPI-positive puncta in ring.
Compatibility
Supported
SA-β-gal + morphometry, SA-β-gal + DNA damage foci (γH2AX, 53BP1), CCF detection, p21/p16 intensity quantification.
Not yet supported
EdU-negative gating (requires combining with proliferation assay in a single workflow), SASP secretion quantification.
1 Dependent on sample quality
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