Mitochondrial Membrane Potential — Endpoint
Measures mitochondrial polarization or depolarization from endpoint TMRM, TMRE, or JC-1 fluorescence images.
- Ch1: DAPI or Hoechst
- Ch2: TMRM or TMRE fluorescence, or JC-1 monomer channel
- Optional Ch3: JC-1 aggregate channel or mitochondrial mass marker
- Mean potential-sensitive intensity per cell
- JC-1 red/green ratio per cell
- % depolarized cells
- Potential signal normalized to mitochondrial mass marker
- Population intensity or ratio distribution
Everything you need for mitochondrial membrane potential — endpoint
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Upload your images and get publication-ready results. No coding or scripting needed.
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Segment cells
Measure potential-sensitive fluorescence in each cell or mitochondrial region
Optionally compute a two-channel ratio
Gate polarized and depolarized populations relative to controls.
Compatibility
Ready to run
TMRM, TMRE, JC-1 red/green ratio, potential signal normalized to mitochondrial mass, fixed or single-endpoint live-cell images.
Inquire for support
Absolute membrane-potential calibration, rapid depolarization kinetics, photobleaching correction through time, or simultaneous organelle tracking.
1 Dependent on sample quality
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