Sub-Object Quantification
Counts and measures smaller objects contained within larger parent objects, then aggregates statistics per parent. This enables quantification of organelles per cell, inclusions per nucleus, or vesicles per compartment.
- Ch1: DAPI
- Ch2: parent object channel (e.g., cell body stain)
- Ch3: sub-object channel (e.g., organelle marker)
- Sub-object count per parent
- Total sub-object area per parent
- Mean sub-object intensity
- Sub-object size distribution
Everything you need for sub-object quantification
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Segment parent objects (e.g., cells)
Detect sub-objects within each parent
Measure sub-object count, area, intensity, and circularity per parent.
Compatibility
Supported
Organelles per cell (mitochondrial fragments, lysosomes, endosomes), inclusions per nucleus (nuclear speckles, Cajal bodies), vesicles per compartment (secretory vesicles, endocytic vesicles), dual sub-object sets per parent (e.g., two organelle types with count ratios).
Not yet supported
Sub-object tracking over time, sub-object-to-sub-object spatial relationships within a parent.
1 Dependent on sample quality
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