Multi-Marker Foci Association
Detects puncta in two channels independently and scores their association per cell via count-based colocalization. Used to determine whether foci of one type co-occur with foci of another type within the same cells.
- Ch1: DAPI
- Ch2: foci marker A
- Ch3: foci marker B
- Foci count per channel per cell
- Count ratio of marker A to marker B per cell
- % cells positive for both markers
Everything you need for multi-marker foci association
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Segment cells
Detect foci in Ch2 and Ch3 independently
Count each type per cell and compute ratios. To determine which individual foci from marker A are positive for marker B: detect spots in Ch2 and measure Ch3 intensity in those spots.
Compatibility
Supported
Telomere-associated DNA damage foci (γH2AX + telomere FISH), autophagosome-mitochondria association (LC3 + TOM20 for mitophagy), dual-probe FISH co-expression (% cells expressing gene A and gene B), PLA + cell type marker, any two-channel puncta comparison.
Not yet supported
Per-spot spatial distance measurement between two spot sets, 3D spot association.
1 Dependent on sample quality
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