Cytosolic Nucleic Acid Sensing (Innate Immunity)
Detects cytoplasmic DNA or cGAS/STING puncta as markers of innate immune activation.
- Ch1: DAPI
- Ch2: anti-dsDNA or cGAS IF
- Optional Ch3: STING or p-IRF3
- Cytoplasmic puncta count per cell
- CCF count
- % cells with cytoplasmic DNA
- Marker intensities
Everything you need for cytosolic nucleic acid sensing (innate immunity)
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How it runs
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Segment nuclei from DAPI
Create cytoplasmic ring (dilate
Subtract nuclei)
Detect DAPI-bright puncta or cGAS foci in the cytoplasmic region only
Count per cell.
Compatibility
Supported
CCF detection (DAPI-positive cytoplasmic bodies), cGAS puncta, STING puncta, combined with γH2AX foci for integrated DNA damage + innate immunity readout.
Not yet supported
Live-cell cGAS dynamics, pathway activation kinetics.
1 Dependent on sample quality
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