DNA Damage & Genotoxicity (Foci)
Quantifies DNA double-strand break burden by counting damage-response foci per nucleus.
- Ch1: DAPI
- Ch2: γH2AX or 53BP1 (IF)
- Optional Ch3: telomere FISH probe (for TAF/TIF)
- Optional Ch4: additional damage marker
- Foci count per nucleus
- Foci intensity
- Foci area. For TAF: Dual-channel foci colocalization ratio per cell
Everything you need for dna damage & genotoxicity (foci)
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Segment nuclei from DAPI
Detect foci in damage marker channel
Count and measure per nucleus. For TAF/TIF: detect spots in both γH2AX and telomere channels independently, count per cell, compute colocalization ratio.
Compatibility
Supported
γH2AX foci, 53BP1 foci, RAD51 foci, TAF/TIF (telomere-associated foci via count-based colocalization), cytoplasmic chromatin fragments (CCF — see Senescence).
Not yet supported
Comet assay (different imaging modality), live-cell DNA damage kinetics.
1 Dependent on sample quality
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