Transwell / Boyden Migration & Invasion
Counts and measures cells that have migrated through a porous membrane, with or without a matrix barrier.
- Brightfield or fluorescence image of the membrane underside
- Nuclear stain such as DAPI, Hoechst, crystal violet, or another migrated-cell stain
- Optional cell-type marker for population-specific migration
- Migrated or invaded cell count
- Cells per unit membrane area
- Total stained-cell area
- % invasion relative to migration control
- Marker-positive migrated-cell count
Everything you need for transwell / boyden migration & invasion
Automated, reproducible, and code-free analysis in the cloud
Cloud-based
No software to install, no GPU required. Run analysis from any browser on any device.
Fully automated
Upload your images and get publication-ready results. No coding or scripting needed.
Reproducible
Every analysis is logged and reproducible. Share protocols with collaborators instantly.
How it runs
Cytely automates the image analysis so you can focus on the science.
Define the membrane analysis region
Segment migrated cells
Exclude pores, debris, and edge artifacts
Count cells and compute density or stained area
Normalize across fields, inserts, or controls.
Compatibility
Ready to run
Transwell migration, Boyden-chamber migration, Matrigel-coated invasion, chemotaxis endpoint counting, fluorescence- or chromogenic-stained membranes, population-specific migration with identity markers.
Inquire for support
Migration trajectories, velocity, directional persistence, or movement through a true 3D matrix.
1 Dependent on sample quality
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