Gene Knockdown & Overexpression Validation
Validates loss or gain of target-protein expression by measuring per-cell fluorescence and the fraction of responding cells.
- Ch1: DAPI
- Ch2: target protein or reporter
- Optional Ch3: transfection or cell-identity marker
- Optional Ch4: downstream phenotype marker
- Target intensity per cell
- % depleted or overexpressing cells
- Target intensity within transfected cells
- Co-expression with downstream markers
Everything you need for gene knockdown & overexpression validation
Automated, reproducible, and code-free analysis in the cloud
Cloud-based
No software to install, no GPU required. Run analysis from any browser on any device.
Fully automated
Upload your images and get publication-ready results. No coding or scripting needed.
Reproducible
Every analysis is logged and reproducible. Share protocols with collaborators instantly.
How it runs
Cytely automates the image analysis so you can focus on the science.
Segment cells
Measure target and optional perturbation-marker intensities
Gate the relevant population
Compare target-expression distributions against controls.
Compatibility
Ready to run
siRNA/shRNA knockdown, CRISPR knockout marker validation, knock-in reporter validation, plasmid overexpression, inducible-expression systems at a fixed endpoint.
Inquire for support
Direct genotyping, transcript quantification without an imaging probe, temporal induction kinetics.
1 Dependent on sample quality
Ready to automate your gene knockdown & overexpression validation analysis?
Get started for free or book a demo to see how Cytely works with your data.
Trusted by leading research institutions
Hundreds of researchers use Cytely to accelerate discovery
Used by researchers at
Simple, transparent pricing
Start free. Upgrade when you need more.
Free
For exploration and learning
$0
Free forever
- 1 monthly analysis credit
- Unlimited public datasets
- Community support
Individual
For active researchers
From $299
per month
- 10+ monthly analysis credits
- Image analysis support
- Priority email support
Lab / Team
For collaborating teams
Custom
Contact us
- 5+ seats included
- Shared folders and templates
- Dedicated support
Loved by researchers
Hear from researchers who use Cytely every day.
The data-driven approach that Cytely offers is a dream to an experienced microscopist like me.
This completely removes bias. The price is negligible when you consider the time spent on image analysis.
It's just amazing what can be done today! Cytely does all the work for me!
Cytely makes me 10 times faster. It saves hours on every experiment.
The insight we gained from Cytely was a completely transformative experience. It allowed us to redesign our experiment and finally move the project forward.
Cytely dramatically improved our timelines while enhancing data reliability through automated, unbiased analysis and significantly larger sample sizes.
With Cytely, we instantly analyzed thousands of cells to precisely identify which were transfected, infected, or both. What had been a three-year roadblock was resolved in our first session.
Curious about this assay?
Book a quick call and our team will help you set up your analysis.
Explore more assays
Other assay needs?
Talk to our image analysis specialists. We're adding new assays regularly.