Clonogenic Survival & Colony Formation
Counts discrete colonies and measures colony size and morphology to quantify reproductive survival after treatment.
- Brightfield or fluorescence image containing stained colonies
- Optional nuclear or whole-colony stain
- Colony count per well or field
- Colony area and equivalent diameter
- Colony circularity and intensity
- % colonies above a size threshold
Everything you need for clonogenic survival & colony formation
Automated, reproducible, and code-free analysis in the cloud
Cloud-based
No software to install, no GPU required. Run analysis from any browser on any device.
Fully automated
Upload your images and get publication-ready results. No coding or scripting needed.
Reproducible
Every analysis is logged and reproducible. Share protocols with collaborators instantly.
How it runs
Cytely automates the image analysis so you can focus on the science.
Segment colony objects from brightfield or fluorescence
Remove debris by size and morphology
Measure colony count, area, diameter, circularity, and intensity
Optionally classify colonies above a minimum-size threshold.
Compatibility
Ready to run
Crystal violet colony formation, fluorescent colony staining, focus-formation assays, colony count and morphology, endpoint plating-efficiency and surviving-fraction workflows.
Inquire for support
Colony growth kinetics, lineage reconstruction, densely merged colonies that cannot be separated reliably.
1 Dependent on sample quality
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