Lipid Peroxidation & Oxidative Stress (ROS)
Measures cellular oxidative burden via fluorescent ROS indicators.
- Ch1: DAPI
- Ch2: ROS probe (e.g., CellROX, MitoSOX, DHE). For ratiometric probes (BODIPY C11): Ch2: reduced form, Ch3: oxidized form
- Mean fluorescence intensity per cell
- % cells above threshold
- Ratio of oxidized/reduced signal (for ratiometric probes)
Everything you need for lipid peroxidation & oxidative stress (ros)
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Segment cells from DAPI
Measure probe intensity per cell
Classify stressed vs unstressed by intensity gating. For ratiometric probes: measure both channels, compute intensity ratio.
Compatibility
Supported
Single-channel ROS probes (CellROX, MitoSOX, DHE), ratiometric probes (BODIPY C11 red/green ratio), multi-marker panels.
Not yet supported
Live-cell kinetic ROS measurements, mitochondrial-specific ROS with organelle segmentation.
1 Dependent on sample quality
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