Lipid Droplet Quantification
Detects and measures lipid droplets within cells to quantify lipid accumulation.

- Ch1: DAPI
- Ch2: lipid dye (BODIPY 493/503, Oil Red O in fluorescence, Nile Red)
- Optional Ch3: cell membrane or cytoplasm stain
- Droplet count per cell
- Droplet area
- Total lipid area fraction
- Mean droplet intensity
Everything you need for lipid droplet quantification
Automated, reproducible, and code-free analysis in the cloud
Cloud-based
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Upload your images and get publication-ready results. No coding or scripting needed.
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How it runs
Cytely automates the image analysis so you can focus on the science.
Segment cells
Detect droplets in lipid channel
Measure droplet count, area, and intensity per cell.
Compatibility
Supported
BODIPY, Nile Red, LipidTOX staining, brightfield (Oil Red O), multi-channel with additional markers, adipocyte-specific detection and quantification.
Not yet supported
3D droplet volume estimation, droplet fusion/fission tracking.
1 Dependent on sample quality
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