PQBP1 couples HIV-1 capsid recognition to cGAS recruitment through conformational remodeling
Allen DS, Manna A, Beusch CM, Zhang T, Ganser-Pornillos BK, Piacentini J, Oh S, Prabhu S, Mehta A, Gordon D, Pornillos O, Yoh SM, Chanda SK. bioRxiv (preprint)
Innate immune detection of HIV-1 starts when PQBP1 recognizes the incoming viral capsid. This preprint shows that capsid engagement remodels the intrinsically disordered PQBP1 into a cGAS-competent conformation, and maps the distinct regions responsible for capsid binding versus cGAS recruitment. Cell identification and per-cell quantification of cGAS-PQBP1 proximity ligation puncta were performed with Cytely AI image analysis.
