Sample Purity & Quality Control
Identification and quantification of contaminants in cell preparations

- Ch1: DAPI or Hoechst (nuclear stain)
- Ch2: target cell marker
- Optional Ch3: contaminant or debris counterstain
- % pure (target) cells
- Contaminant count and fraction
- Debris and aggregate area per field
- Per-object classification (target vs. contaminant vs. debris)
Everything you need for sample purity & quality control
Automated, reproducible, and code-free analysis in the cloud
Cloud-based
No software to install, no GPU required. Run analysis from any browser on any device.
Fully automated
Upload your images and get publication-ready results. No coding or scripting needed.
Reproducible
Every analysis is logged and reproducible. Share protocols with collaborators instantly.
How it runs
Cytely automates the image analysis so you can focus on the science.
Segment all objects from the nuclear and marker channels
Classify each as target cell, contaminant, or debris using size, shape, and marker intensity
Report sample purity as the fraction of target cells, with every flagged object linked back to its image.
Compatibility
Ready to run
Single-cell suspension QC, dissociated primary tissue purity, cell-line cross-contamination checks, debris and aggregate screening, and any preparation where target cells must be separated from contaminating objects.
Inquire for support
Mycoplasma detection, microbial species identification, and sterility or endotoxin testing.
1 Dependent on sample quality
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The data-driven approach that Cytely offers is a dream to an experienced microscopist like me.
This completely removes bias. The price is negligible when you consider the time spent on image analysis.
It's just amazing what can be done today! Cytely does all the work for me!
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The insight we gained from Cytely was a completely transformative experience. It allowed us to redesign our experiment and finally move the project forward.
Cytely dramatically improved our timelines while enhancing data reliability through automated, unbiased analysis and significantly larger sample sizes.

With Cytely, we instantly analyzed thousands of cells to precisely identify which were transfected, infected, or both. What had been a three-year roadblock was resolved in our first session.
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