Apoptosis (Annexin V / PI)
Classifies cells into viable, early apoptotic, late apoptotic, and necrotic populations using dual-marker staining and quadrant gating.
- Ch1: DAPI or Hoechst
- Ch2: Annexin V (early apoptosis marker)
- Ch3: propidium iodide (late apoptosis / necrosis marker)
- Four-population classification (viable, early apoptotic, late apoptotic, necrotic)
- % cells per population
- Per-cell Annexin V and PI intensities
Everything you need for apoptosis (annexin v / pi)
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Segment cells
Measure Annexin V and PI intensity per cell
Apply interactive 2D scatter plot gating (Annexin V vs PI) to classify cells into four quadrants, analogous to flow cytometry.
Compatibility
Supported
Annexin V / PI, Annexin V / 7-AAD, any dual live/dead marker combination with quadrant gating.
Not yet supported
Time-resolved apoptosis kinetics, caspase activity reporters.
1 Dependent on sample quality
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