Multi-Nuclei Detection

Identifies multinucleated cells within a population for downstream analysis or targeted re-acquisition at higher magnification.

Multi-Nuclei Detection illustration
Input channels
  • Ch1: DAPI (nuclear stain)
  • Optional Ch2: cell body stain (to define cell boundaries)
Output metrics
  • Number of nuclei per cell
  • % multinucleated cells
  • Re-acquisition coordinates for targeted high-resolution imaging

Everything you need for multi-nuclei detection

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How it runs

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01

Segment nuclei from DAPI

02

Segment cell bodies from Ch2 or by dilation

03

Count nuclei per cell body

04

Flag cells with multiple nuclei.

Compatibility

Supported

Multinucleated cell detection, polyploidy screening, fusion assays.

Not yet supported

Automated re-acquisition triggering (instrument-level capability, not analysis).

1 Dependent on sample quality

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