Cell Cycle Analysis
Categorizes cells into G0/G1, S/G2, and M phases using cell morphology and DNA stain intensity, preserving spatial context unlike flow cytometry.
- Ch1: DAPI or Hoechst (DNA content)
- Optional Ch2: cell body stain for morphology
- Cell cycle phase classification per cell
- % cells per phase
- DNA content (integrated DAPI intensity) per cell
- Nuclear area per cell
Everything you need for cell cycle analysis
Automated, reproducible, and code-free analysis in the cloud
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How it runs
Cytely automates the image analysis so you can focus on the science.
Segment nuclei
Measure integrated DAPI intensity (DNA content proxy) and nuclear area per cell
Apply interactive 2D gating (DNA content vs area) to classify G0/G1, S, G2/M populations.
Compatibility
Supported
DAPI/Hoechst DNA content, combined with EdU for S-phase confirmation, combined with phospho-Histone H3 for M-phase.
Not yet supported
Traditional DNA content histogram gating (PI flow-style), sub-G1 apoptosis detection.
1 Dependent on sample quality
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