Transfection Efficiency
Quantifies the fraction of cells expressing one or more transfected constructs.
- Ch1: DAPI
- Ch2: fluorescent reporter from plasmid A (e.g., GFP)
- Optional Ch3: fluorescent reporter from plasmid B (e.g., mCherry)
- % transfected cells (single or dual positive)
- Reporter intensity per cell
- Total cell count
Everything you need for transfection efficiency
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Segment nuclei from DAPI
Measure reporter channel intensity per cell
Classify transfected/untransfected by intensity gating. For dual plasmids: gate on both channels to identify single-positive and double-positive populations.
Compatibility
Supported
Single plasmid, dual plasmid co-transfection, viral transduction efficiency, any fluorescent reporter construct.
Not yet supported
Transient vs stable expression discrimination.
1 Dependent on sample quality
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