Multi-Marker Foci Association

A multi-channel acquisition for detecting multiple DNA-damage foci markers at the single-cell level. Cytely segments nuclei, detects foci across channels, and reports per-cell foci count, intensity, and area in cells exposed to a DNA-damaging agent.

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Attribution

ContributorsAris Polyzos

InstitutionLawrence Berkeley National Laboratory

Cite as

These images were provided by Aris Polyzos at Lawrence Berkeley National Laboratory.

Assays in this dataset

Puncta, Foci & Sub-Object Analysis

Multi-Marker Foci Association

Detects puncta in two channels independently and scores their association per cell via count-based colocalization. Used to determine whether foci of one type co-occur with foci of another type within the same cells.

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