Bacteria Internalization - Salmonella Infected HeLa Cells
HeLa cells infected with mCherry-labelled Salmonella enterica, imaged by high-content confocal microscopy. This example dataset shows how to detect and quantify intracellular bacteria at the single-cell level, comparing the number of bacteria per host cell across different pathogen concentrations.
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Methodology
Salmonella enterica-infected HeLa cells imaged on a PerkinElmer Opera LX high-content confocal system (40x air objective, NA 0.6). Three fluorescence channels: DAPI (nuclei, 365 nm), ER-tracker (488 nm), and mCherry-Salmonella (561 nm). Cytely segments host cell nuclei and cytoplasm, then detects bacteria as sub-objects within each cell to report the number of bacteria per host cell across pathogen concentrations.
Attribution
Antoniou, A. N., Powis, S. J., & Kriston-Vizi, J. (2018). High-content screening image dataset and quantitative image analysis of Salmonella infected human cells. Harvard Dataverse, V2. https://doi.org/10.7910/DVN/FYGHFO
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