Spot & Foci Detection - Intracellular Sub-Object Counting
A multi-channel high-content image dataset for intracellular spot and foci detection. This example dataset shows how to detect and quantify intracellular sub-objects at the single-cell level with Cytely, comparing sub-object counts per cell across different experimental conditions.
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Watch how this dataset was analyzed end to end in Cytely.
Methodology
High-content fluorescence images with a nuclear stain and one or more marker channels. Cytely segments cell nuclei and cytoplasm, detects sub-objects (spots/foci) within each cell, and reports sub-object counts per cell for comparison across experimental conditions.
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