Nuclear Translocation Benchmark (BBBC014v1)
A 2-channel acquisition (DAPI nuclear counterstain + FITC NF-κB signal) from the BBBC014 benchmark: cytoplasm-to-nucleus translocation of the transcription factor NF-κB in MCF7 and A549 cells in response to TNF-α. Cytely segments nuclei and the surrounding cytoplasm and reports the per-cell nuclear/cytoplasmic NF-κB intensity ratio, which rises dose-dependently with TNF-α.
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We used image set BBBC014v1 provided by Ilya Ravkin, available from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012; doi: 10.1038/nmeth.2083]. https://bbbc.broadinstitute.org/BBBC014 NF-κB cytoplasm-to-nucleus translocation in MCF7 (breast adenocarcinoma) and A549 (alveolar basal epithelial) cells in response to TNF-α. License: CC BY 3.0 Unported.
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