Multiplex Imaging Analysis
A 5-channel Cell Painting acquisition of human U2OS cells from a compound-profiling screen of ~1,600 bioactive compounds. Cytely segments nuclei, cytoplasm, nucleoli, concanavalin-A puncta, and mitochondria, then reports per-cell morphology and object counts across small-molecule treatment groups.
LicenseCC0 (Public Domain)
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Methodology
5-channel Cell Painting of human U2OS cells. Hoechst 33342 (nuclei); concanavalin A / FITC (endoplasmic reticulum); SYTO 14 (RNA / nucleoli); WGA + phalloidin / Texas Red (Golgi and cell membrane); MitoTracker Deep Red (mitochondria).
Attribution
ContributorsGustafsdottir SM, et al.
InstitutionBroad Bioimage Benchmark Collection
Year2013
Cite as
We used image set BBBC022v1 [Gustafsdottir et al., PLOS ONE, 2013; doi: 10.1371/journal.pone.0080999], available from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012; doi: 10.1038/nmeth.2083]. https://bbbc.broadinstitute.org/BBBC022 Released under CC0 (public domain); Anne Carpenter has waived all copyright to BBBC022v1.
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