Cell Morphology - Pluripotent Stem Cell Differentiation States
A multi-channel high-content image dataset of human pluripotent stem cells. This example dataset shows how to explore and distinguish cell subpopulations by morphology with Cytely: segmenting nuclei and cytoplasm, measuring morphological features and signal intensity, and using morphological gating to isolate and compare distinct differentiation states.
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Watch how this dataset was analyzed end to end in Cytely.
Methodology
Multi-channel fluorescence images of human pluripotent stem cells. Cytely segments nuclei and cytoplasm, extracts per-cell morphological features and signal intensities across channels, and applies morphological gating to isolate and compare subpopulations corresponding to distinct differentiation states.
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