Blood Cell Analysis - Neutrophils

Analyzing cell populations in whole blood samples containing mixed populations of leukocytes, platelets, and red blood cells (RBCs). This dataset demonstrates rapid identification and quantification of different blood cell types at the single-cell level through scatter plots and intuitive gating. Successfully identifies neutrophils (57%), platelets (11%), and RBCs (12%) from a heterogeneous sample.

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Blood Cell Analysis - Neutrophils — 1,130 detections

Data points1,130

LicenseAcademic Use Only

Methodology

Peripheral blood obtained from healthy volunteer by venipuncture. Neutrophils isolated by density gradient centrifugation using Ficoll-Paque. Cells stained with CellMask™ Green Plasma Membrane Stain (2 µM), CellTracker™ Deep Red (1 µM), and Hoechst 33342 nuclear stain (5 µg/mL).

Usage examples

Visualizing heterogeneity across mixed cell populations. Identifying populations (neutrophils, platelets, RBCs) using scatter plots. Gating to isolate neutrophil subsets. Quality control of blood cell preparations.

Technical notes

Three fluorescent channels used: Hoechst 33342 (350/461 nm) for nuclei, CellMask Green (522/535 nm) for membrane, and CellTracker Deep Red (630/650 nm) for cytoplasm. Images acquired using Nikon TI2 widefield microscope.

Related resources

Application Note - Identifying Contaminants in Neutrophil Preparations

Analyzing cell populations in whole blood is a fundamental yet challenging task. Even after standard preparation steps, samples often contain mixed populations of leukocytes, platelets, and red blood cells (RBCs). Cytely enables researchers to rapidly identify and quantify these populations at the single-cell level. Through scatter plots and intuitive gating, different blood cell types can be distinguished and characterized in an unbiased, interactive workflow. Key features demonstrated in this note: Visualizing heterogeneity across a mixed cell population Identifying populations (neutrophils, platelets, RBCs) using scatter plots Gating to isolate neutrophil subsets

How to identify different blood cell types in your sample (neutrophils, RBCs, platelets) without multiple antibodies or elaborate protocols

Here we look at a basic blood sample stained for nuclei, cytosol, and membrane. We easily gated out RBCs and platelets by their lack of nuclear signal and smaller size, while neutrophils popped out with their strong nuclear and cytosolic staining. Ordinarily, you’d need multiple antibodies or RBC lysis to achieve this separation, but with Cytely, you can do it in a single workflow, preserving your sample’s spatial context and completing the entire analysis in a matter of minutes.

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