Immunology Lab, Lund University

From false positives to MOA-verified hits with visual phagocytosis screening.

Challenge

  • Flow cytometry stripped adherent macrophages (changing their biology) and produced false positives from dye transfer
  • Cannot verify true phagocytosis vs. surface contamination
  • Need for complex coding to automate

Solution

  • Image-based analysis preserves adherent cell biology with visual verification of internalization
  • Browser-based interface - no programming required
  • Population gating distinguishes true double-positives from artifacts

Key Benefits

Caught
artifacts flow cytometry missed
Linked
binding to function—visual proof of mechanism
Eliminated
bioinformatics bottleneck

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