Lysosomes sit at the center of neurodegeneration, lysosomal storage disease, mTOR signaling, and a fast-growing pipeline of autophagy modulators. See the analysis in action: https://youtube.com/watch?v=FHkNHhXVEsg
Lysosomes sit at the center of neurodegeneration, lysosomal storage disease, mTOR signaling, and a fast-growing pipeline of autophagy modulators.
The organelle that used to be the end of the pathway is now one of the most measured compartments in CNS and rare disease discovery.
The question every one of those programs asks is the same: are the lysosomes actually acidic?
Cytely pairs a lysosomal marker with pH-sensitive dyes, segments cells, detects every lysosome, and scores the acidic fraction per cell.
How the assay runs
Segment cells → detect lysosomes in lysosomal marker channel → measure pH-dye intensity per lysosome → classify acidic vs neutral → report count and % acidic per cell.
What you provide
- Ch1: DAPI
- Ch2: lysosomal marker (e.g., LAMP1, LysoTracker)
- Ch3: pH-sensitive dye (e.g., LysoSensor, pHrodo)
What you get
- Lysosome count per cell
- % acidic lysosomes per cell
- Mean lysosomal intensity per channel
- Total lysosomal area
Ready to run
LysoTracker + LysoSensor, LAMP1 IF + pHrodo, any lysosomal marker + pH indicator combination.
Inquire for support
Live-cell lysosomal trafficking, lysosomal pH calibration curves.