A drug is useless if it can't get inside the cell. This is the central challenge for lipid nanoparticles, cell-penetrating peptides, antibody-drug conjugates, and RNA… See the analysis in action: https://youtube.com/watch?v=1qo3xSGYcl8
A drug is useless if it can't get inside the cell. This is the central challenge for lipid nanoparticles, cell-penetrating peptides, antibody-drug conjugates, and RNA therapeutics, the fastest-growing modalities in pharma. Every delivery team needs to know: how much got in, and into how many cells?
Cytely quantifies uptake at the single-cell level across conditions in seconds. Interactive gating lets you filter artifacts and isolate genuinely positive cells with visual verification. Replace "Nanoparticles" with your LNP-encapsulated mRNA or your cell-penetrating peptide and the workflow is identical.
How the assay runs
Segment cells → measure intracellular fluorescence intensity per cell → compare across conditions.
What you provide
- Ch1: DAPI
- Ch2: fluorescently labeled peptide, toxin, or compound (e.g., pHrodo, Alexa-labeled)
What you get
- Mean intracellular fluorescence per cell
- % cells with uptake
Ready to run
Cell-penetrating peptide uptake, cholera toxin binding and internalization, transferrin uptake, fluorescent glucose uptake (2-NBDG), fatty-acid / lipid uptake, labeled LDL uptake (e.g., DiI-LDL), exosome / extracellular vesicle (EV) uptake, nanoparticle uptake, receptor-mediated endocytosis, any fluorescently labeled compound uptake assay.
Inquire for support
Concentration-dependent uptake profiles, endosomal escape quantification, uptake kinetics (timelapse).