When the same cell-based assay runs again and again, and more than one person needs to run it, check it or share it. CellProfiler and ImageJ/Fiji are free, open-source tools that work well for one-off projects, method development, and cases where analysis has to stay on your own hardware. They tend to become a bottleneck when the analysis depends on the one person who built the pipeline or wrote the macro.

Cytely is a good alternative if you want to:

  • Let anyone on the team build and run an analysis, without pipeline or scripting expertise
  • Get the same result on every batch, whoever runs it
  • Check any population against the cells behind it
  • Share the full analysis with colleagues, not only exported tables

Cytely does this with ready assays, an AI workflow builder and a visual no-code builder, gating linked to every cell, and a shared cloud workspace. If your assay needs something new, we build a custom workflow for you at no cost.

For the full comparisons, see CellProfiler alternative, ImageJ and Fiji alternative, or the side-by-side comparison table.