In this short video I will walk you through the results of an analysed phagocytosis sample. The sample consists of THP-1 cells who have been given fluorescent beads, opsonized with an antibody.

Experimental Design

This tutorial demonstrates the analysis workflow for a phagocytosis assay using THP-1 cells. The experimental setup involves:

  • THP-1 cells - human monocytic cell line commonly used for phagocytosis studies
  • Fluorescent beads - opsonized with antibodies to enhance phagocytic uptake

Analysis Workflow

The video walks through the complete analysis pipeline in Cytely's Data Explorer, showing how to:

  • Visualize the spatial distribution of cells and phagocytic events
  • Quantify bead uptake per cell
  • Analyze phagocytic efficiency across the population
  • Filter and gate specific cell populations
  • Export quantitative data for further statistical analysis

Key Benefits

This application note demonstrates how Cytely enables rapid, unbiased quantification of phagocytosis experiments - transforming manual, time-consuming analysis into an automated, reproducible workflow.